Thursday, April 1, 2010

beauti health & tips for urdu


Amid the busy lifestyles, long working hours, jam-packed schedules most of the times, most of us end up ignoring our health. So, to give you a wake-up call to rise healthier and live longer, we bring to you ten ways to live healthy....


Go for exercise Regular exercising helps in maintaining or gradually increasing the strength and endurance of heart and other muscles. An exercise program may include a variety of aerobic activities, such as walking, swimming and cycling. If one has a busy and sedentary lifestyle, a walk of 5 minutes after every hour is necessary.

Healthy eating - One may unlock the door to healthy life by eating healthy. Healthy eating never stands for eating less or dieting, but it’s all about eating a nutritious and a well balanced diet. A healthy diet is one which includes a good combination of fruits, vegetables, dairy products. One should go for 4-5 servings of fruits and vegetables per day to stay fit. If you are really interested in maintaining a good health, then prefer not to miss your breakfast because it provides with the energy or fuel required by the body after a gap of long night.

Go for water - Drink plenty of fresh water, to keep your body healthy. One can have 4 liters of liquid per day. (Heart patients who are on fluid restrictive diets, or have a problem with fluid retention should consult a physician before increasing their consumption of fluids)

Salt restriction - Modest salt restriction, which means limiting common salt intake to 6 grams per day, lowers the blood pressure. This effect is particularly important for those who have severe hypertension to begin with. They respond better to salt restriction. Salt restriction is also especially effective in elderly and obese hypertensive patients. The magnitude of reduction in the risk of stroke and coronary heart disease by salt restriction alone is estimated to be 22 percent and 16 percent respectively. Please note, a salt free diet is not advisable in most instances. In fact, very low salt intake may cause marked fall in blood pressure, leading to giddiness and transient impairment of consciousness. Salt restriction may be somewhat relaxed during peak summer when considerable loss of sodium may occur via sweating.

Sleep well - Having proper and apt sleep is one of the most important rationales to keep you fit and fine. It helps you to mark a provisional end to your fears and apprehensions. An adult should have a minimum sleep for 7-8 hours while a child should have 10 hours of sleep.

Maintain ideal weight - Carrying a lot of extra weight as fat can greatly affect your health and increases the risk of life-threatening conditions such as coronary heart disease and diabetes. One can easily calculate their ideal body weight through their Body Mass Index (BMI).
BMI = [Weight (in kilograms) / Height (in inches) * 2] * 703
If your BMI lies in the range of 19 to 25 it means you are healthy, below that range is underweight while from 25 29 is overweight and 30 means obese. If you are overweight or obese, start by making small, but healthy changes to what you eat, and try to become more active.

Manage your stress levels Hectic lifestyles accompanied with ever rising aspirations has become a major cause of stress in our lives. If not managed in a proper way, stress can give rise to emotional, physical, and psychological problems and can even take a toll on your heart. To manage stress one can opt for simple things which help in distressing like getting a pet in their lives, or sparing time for their hobbies, or playing with kids.

Monitor your blood pressure and cholesterol levels - People with high blood pressure run a higher risk of having a stroke or a heart attack. It is ideal to monitor the blood pressure daily and report if there is any change in the reading. Keeping blood pressure on the lower side helps to decrease the strain on the heart.

Keep a tab on your Alcohol intake It is observed that hypertension is more common in people who consume more than 1-2 ounces of alcohol every day. Heavy alcohol intake may increase the BP acutely. Increase in blood pressure may persist up to as long as 1 week after alcohol intake. Reduction in alcohol intake from 440 ml to 66 ml per day has been found to reduce systolic blood pressure by 5 mm Hg and diastolic pressure by 3 mm Hg. Therefore, hypertensive patients should avoid more than 1-2 drinks in a day. Some people, especially women may be more sensitive to alcohol. They should abstain from alcoholic drinks.

Go for preventive health checkups You do not need to go to doctor only for medical emergencies. In times of slow resistance, unhealthy lifestyles we should realize the importance of timely medical check-ups. We should be regular with our check-ups, before there is a serious condition
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breast cancer cells evade therapeutic attacks


Professor Didier Picard’s team in Switzerland uncovers a mechanism of resistance of tumour cells to the widespread treatment of the so-called hormone-dependent types

Tumour cells depend upon estrogens to survive and proliferate in about 70% of all breast cancer cases. The most frequently used treatment to fight this variety of tumours relies on anti-estrogens such as tamoxifen. However, resistance to this type of therapy develops in more than 30% of the patients. Understanding the mechanisms involved in the appearance of resistance to tamoxifen is thus essential to develop new therapeutic approaches. The research done by the team of Didier Picard, professor at the University of Geneva (UNIGE), provides key answers in the 1st of April 2010 edition of Genes & Development. Their study reveals how cancer cells become impervious to the drug by activating a specific biochemical cascade. The latter, normally triggered by a chemical messenger called cyclic AMP, is permanently stimulated in cells refractory to the treatment.
Breast cancer, which is characterized by an enhanced and anarchic proliferation of mammary cells, is one of the main causes of cancer-related mortality in women. In over two thirds of the cases, estrogens play an active part in the progression of the disease, due to the presence of receptors for this hormone in the nucleus of malignant cells. Estrogens indeed modulate the expression of genes necessary to cell survival and proliferation through their interaction with these receptors.
The most frequent treatment for this type of breast cancer, called hormone-dependent, relies on anti-estrogens such as tamoxifen, which block tumour cell growth by inhibiting the activity of the receptor. However, the cancer eventually becomes resistant to the therapy in more than 30% of the patients.
The second messenger’s key role
“Estrogen receptors, notably the one called ERI±, are also activated by other molecules that operate in an indirect way, without interacting with them”, explains Didier Picard, professor at the Faculty of Sciences of UNIGE. “We aim precisely at understanding the mechanisms involved in the indirect stimulation of ERI± in cancer cells”.
Researchers from his team have studied a molecule, cyclic AMP, which is able to convey various types of signals within the cell. Called a “second messenger”, this molecule acts as an intermediary to transmit information between the exterior and the interior of the cell. Different growth factors, neurotransmitters or hormones, incapable of crossing the cell barrier, thus communicate their message by activating intracellular signalling pathways via cyclic AMP.
A novel mode of regulation
“During this project, we have studied how cyclic AMP could turn on ERI± in breast cancer cells in the absence of estrogens”, says lead author Sophie Carascossa. In this case, the second messenger triggers a specific biochemical signalling cascade in which a protein called CARM1 is involved. The protein then binds ERI± and this in turn activates the receptor. “The interaction with CARM1 occurs in a domain of the receptor near the one that normally binds estrogens. This mode of regulation was completely unknown until now”, specifies the scientist. The study also reveals that the signalling pathway is stimulated in a constitutive way in malignant cells resistant to tamoxifen, thus evading control in these cells.
Although the molecular mechanisms implicated in the resistance process are probably numerous, the interaction between CARM1 and ERI± could represent a promising therapeutic target in the long run.

approach for rapid sexing embryo


The ability to determine animal gender could improve efficiency of animal production and animal selection. In dairy systems, sex determination would permit the specific production of either male or female embryos. In commercial dairy operations, female embryos of high genetic merit would be preferred for use as replacement stock. Artificial insemination organizations making planned matings would prefer males for their potential value as sires. A reliable method of sex determination is required for such a system.
Several methods have now been reported for mammalian sex determination as following: (i) Using polymerase chain reaction (PCR) to amplify a Y-chromosome-specific repeat sequence, DYZI, that is present 500-8000 times on the Y chromosome. The presence of this amplified target sequence indicates the presence of the Y-specific DNA sequence in the sample DNA and, hence, indicates that the animal is male. A disadvantage of this technique is that the absence of the amplified target sequence may be due either to the absence of the sequence in the sample DNA or to a failed reaction, (ii) Second method involves PCR-based genotyping. In brief, a pseudo-autosomal region (common to both X and Y chromosomes) present in both X and Y chromosomes is amplified. These homologous regions are called ZFY (in the Y chromosome) and ZFX (in the X chromosome). The amplified product is then digested with restriction enzymes that take advantage of restriction enzyme fragment length polymorphisms between ZFY and ZFX. The digested DNA is separated via electrophoresis. The ZFX and the ZFY DNA are identified by their different digestion patterns, (iii) Another PCR-mediated approach uses three sets of primers for sex determination of pre-implantation bovine embryos. As a positive control, the first set of primers amplifies a bovine-specific satellite DNA sequence. Thus, both male and female samples have this amplified target sequence. Two pairs of primers that recognize repetitive bovine Y-chromosome-specific sequences are then used in a PCR amplification reaction and (iv) Lastly and recently, novel technique was reported and developed. The main objective of our study is to develop improve in rapid sexing method for preimplantation embryos of bovine using loop-mediated isothermal amplification (LAMP) reaction. Embryo sexing has been recognized to control effectively the sex of offspring in the embryo transfer industry. This rapid and simple detection system was improved for bovine embryo sexing by adding ethidium bromide (EB) or CuSO4 at different concentrations to the product of LAMP reaction. The result of these additions was a colour change and a precipitate. It could be employed as an alternative method in the detection of the reaction products in place of the time consuming electrophoresis or the turbidity meter. This study showed that the present method can be applied in breeding programs to facilitate manipulation of the sex ratio of offspring. In conclusion, the present procedure without turbidity meter and electrophoresis was reliable and applicable for sexing both the bovine and buffalo embryos.
The sex determination assay developed in this study will be a useful adjunct to the technologies of embryo transfer and cloning. A sex determination assay of this kind will also facilitate scientific studies of gender differences in embryonic and fetal gene expression and development.



















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